Acquisition of accurate data from intramolecular quenched fluorescence protease assays
نویسندگان
چکیده
منابع مشابه
Acquisition and Accurate Use of English Articles by Persian Speakers
This study was conducted with the purpose of examining Persian speakers’ article acquisition and use with reference to Ionin, Ko and Wexler’s (2004) model, which is based on the prediction of Fluctuation Hypothesis (FH) that EFL learners of [-article] languages, like Persian, make erroneous article use in [+definite, -specific] and [-definite, +specific] contexts. From among the students of an ...
متن کاملAcquisition and Accurate Use of English Articles by Persian Speakers
This study was conducted with the purpose of examining Persian speakers’ article acquisition and use with reference to Ionin, Ko and Wexler’s (2004) model, which is based on the prediction of Fluctuation Hypothesis (FH) that EFL learners of [-article] languages, like Persian, make erroneous article use in [+definite, -specific] and [-definite, +specific] contexts. From among the students of an ...
متن کاملHighly sensitive and adaptable fluorescence-quenched pair discloses the substrate specificity profiles in diverse protease families
Internally quenched fluorescent (IQF) peptide substrates originating from FRET (Förster Resonance Energy Transfer) are powerful tool for examining the activity and specificity of proteases, and a variety of donor/acceptor pairs are extensively used to design individual substrates and combinatorial libraries. We developed a highly sensitive and adaptable donor/acceptor pair that can be used to i...
متن کاملRadioactivity-synchronized fluorescence enhancement using a radionuclide fluorescence-quenched dye.
We demonstrate the first evidence of radioactivity-synchronized fluorescence quenching of a near-infrared light-emitting dye by a radionuclide, (64)Cu, and subsequent fluorescence enhancement upon (64)Cu decay to the daughter isotopes (64)Ni and (64)Zn. The dynamic switch from high radioactivity and low fluorescence to low radioactivity and high fluorescence is potentially useful for developing...
متن کاملComparison of different fluorescence fluctuation methods for their use in FRET assays: monitoring a protease reaction.
We compare the accuracy of a variety of Fluorescence Fluctuation Spectroscopy (FFS) methods for the study of Förster Resonance Energy Transfer (FRET) assays. As an example, the cleavage of a doubly labeled, FRET-active peptide substrate by the protease Trypsin is monitored and analyzed using methods based on fluorescence intensity, Fluorescence Correlation Spectroscopy (FCS) and Fluorescence In...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2017
ISSN: 0003-2697
DOI: 10.1016/j.ab.2017.01.020